Detection of Recombinant Human Erythropoietin in Urine by Isoelectric Focusing
نویسندگان
چکیده
منابع مشابه
Detection of recombinant human erythropoietin in urine by isoelectric focusing.
BACKGROUND Doping with erythropoietic proteins such as recombinant human erythropoietin (rHuEPO) and darbepoetin alfa is a serious issue in sport. There is little information on the time course of detection of rHuEPO in urine and on methods to evaluate electrophoresis-based data. METHODS We used a recently described isoelectric focusing method for detecting rHuEPO and endogenous EPO in urine ...
متن کاملComparison of the isoelectric focusing patterns of darbepoetin alfa, recombinant human erythropoietin, and endogenous erythropoietin from human urine.
rejection by elevated circulating levels of serum intestinal fatty acid binding protein. Surgery 1993;114:206–10. 12. Ascher N, Stock PG, Baumgarder GL, Payne WD, Najarian JS. Infection and rejection of primary hepatic transplant in 93 consecutive patients treated with triple immunosuppressive therapy. Surg Gynecol Obstet 1988;167: 474–84. 13. Hughes VF, Trull AK, Joshi O, Alexander GJM. Monito...
متن کاملDetection of recombinant human erythropoietin in urine for doping analysis: interpretation of isoelectric profiles by discriminant analysis.
The detection in urine of recombinant human erythropoietin (rHuEPO), a hormone misused by endurance athletes as a doping agent, is based on the differentiation of its isoelectric pattern from that of the corresponding natural hormone. Different empirical criteria have been proposed for discriminating the images of the patterns but none of them have been elaborated from a rational statistical ap...
متن کاملAssessing the instability of the isoelectric focusing patterns of erythropoietin in urine.
IEF can be used to differentiate human urinary erythropoietin (uEPO), recombinant human erythropoietin or epoetin (rEPO) and darbepoetin (novel erythropoiesis stimulating protein (NESP)). This is the basis of the method currently used to detect misuse of rEPO and NESP by elite athletes. Recently, an unknown activity has been attributed to some urine samples (denominated 'unstable' urine by the ...
متن کاملIsoelectric Focusing of Human Liver
1. Isoelectric focusing of human liver alkaline phosphatase in a sucrose density gradient with LKB Ampholine as carrier ampholytes is described. 2. Problems due to the chelating properties of the ampholytes and the pH gradient were examined. 3. A reactivation procedure to counter these effects was devised that can probably be used for other alkaline phosphatases. 4. The isoelectric point of hum...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2003
ISSN: 0009-9147,1530-8561
DOI: 10.1373/49.6.901